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Please use this identifier to cite or link to this item: https://hdl.handle.net/11681/6421
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dc.contributor.authorZattau, William C.en_US
dc.creatorAquatic Plant Control Research Program (U.S.)en_US
dc.creatorEnvironmental Laboratory (U.S.)en_US
dc.date.accessioned2016-03-23T19:55:37Zen_US
dc.date.available2016-03-23T19:55:37Zen_US
dc.date.issued1988-04en_US
dc.identifier.govdocTechnical Report A-88-3en_US
dc.identifier.urihttp://hdl.handle.net/11681/6421en_US
dc.descriptionTechnical Reporten_US
dc.description.abstractA survey of the continental United States for pathogens of Myriophyllum spicatum (Eurasian watermilfoil) was conducted. More than 50 water bodies, located in 10 states, were sampled for diseased plants. Sample sites represented a geographic and climatic cross section of aquatic systems in the continental United States, including ponds, lakes, reservoirs, rivers, and canals. At the conclusion of the survey, 792 isolates had been collected from tissue samples and maintained in pure culture. Of these, 462 were bacteria and 330 were fungi; many isolates were duplicates but were retained because they possibly represented different strains of the same species. Lytic enzyme assays indicated that 14 bacterial and 22 fungal isolates produced pectinase or cellulose. Enzyme assays were used to screen for the more promising isolates for additional study as potential biocontrol agents for the management of Eurasian watermilfoil. The 36 isolates were then assayed against healthy sprigs of the target species in test tubes. Results indicated that five fungal isolates should be considered for additional study.en_US
dc.description.sponsorshipAquatic Plant Control Research Program (U.S.)en_US
dc.description.sponsorshipUnited States. Army. Corps of Engineersen_US
dc.description.tableofcontentsPreface......................1 List of Tables......................4 List of Figures......................4 Part I: Introduction......................5 Background......................5 Conventional Control......................6 Biological Control Agents of Eurasian Watermilfoil......................6 Approach......................8 Rationale......................8 Purpose and Objectives......................9 Part II: Materials and Methods......................10 Site Selection......................10 Site Descriptions......................10 Collection of Samples......................13 Processing of Samples......................13 Lytic Enzyme Screening......................16 Inoculum Viability......................16 Test Tube Assay......................16 Reisolation......................17 Identification of Isolates......................18 Part III: Results......................20 Observed Pathology......................20 Isolate Colelction......................20 Lytic Enzyme Assay......................21 Isolate Identification......................21 Inoculum Viability......................21 Test Tube Assay......................21 Part IV: Discussion......................27 Field Observations......................27 Isolate Pathology......................27 Part V: Conclusions and Recommendations......................29 References......................30 Appendix A: Dates and Locations of Collections of Eurasian Watermilfoil......................A1 Appendix B: Ranked Weekly Mean Damage Index Values for Fungal Isolates......................B1 Appendix C: Ranked Weekly Mean Damage Index Values for Bacterial Isolates......................C1 Appendix D: Analysis of Variance of Lytic Enzyme Positive Fungal Isolates......................D1en_US
dc.format.extent47 pages/7.15 MBen_US
dc.format.mediumPDFen_US
dc.language.isoen_USen_US
dc.publisherU.S. Army Engineer Waterways Experiment Stationen_US
dc.relationhttp://acwc.sdp.sirsi.net/client/en_US/search/asset/1043712en_US
dc.relation.ispartofseriesTechnical Report (Aquatic Plant Control Research Program (U.S.)) ; no.Technical Report A-88-3en_US
dc.rightsApproved for public release; distribution is unlimiteden_US
dc.sourceThis Digital Resource was created from scans of the Print Resourceen_US
dc.subjectEurasian watermilfoilen_US
dc.subjectMyriophyllum spicatumen_US
dc.subjectPathogenen_US
dc.subjectSurveyen_US
dc.subjectBacteriaen_US
dc.subjectFungien_US
dc.subjectBiological controlen_US
dc.subjectBiocontrolen_US
dc.subjectAquatic plantsen_US
dc.subjectAquatic weedsen_US
dc.subjectAquatic Plant Control Research Program (U.S.)en_US
dc.titleA survey of the continental United States for pathogens of Eurasian watermilfoilen_US
dc.typeReporten_US
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