1. ERDC Knowledge Core
  2. Engineer Research and Development Center (ERDC)
  3. Cold Regions Research Engineering Laboratory (CRREL) - (2/1/1961 - present)
  4. Publication
  5. Technical Report
Please use this identifier to cite or link to this item: https://hdl.handle.net/11681/45620
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dc.contributor.authorAsenath-Smith, Emily-
dc.contributor.authorJeng, Emily C.-
dc.contributor.authorAmbrogi, Emma K.-
dc.contributor.authorHoch, Garrett R.-
dc.contributor.authorOlivier, Jason L.-
dc.creatorCold Regions Research and Engineering Laboratory (U.S.)-
dc.date.accessioned2022-09-30T13:40:34Z-
dc.date.available2022-09-30T13:40:34Z-
dc.date.issued2022-09-
dc.identifier.govdocERDC/CRREL TR-22-17-
dc.identifier.urihttps://hdl.handle.net/11681/45620-
dc.identifier.urihttp://dx.doi.org/10.21079/11681/45620-
dc.descriptionTechnical Reporten_US
dc.description.abstractAntifreeze proteins (AFPs) allow biological organisms, including insects, fish, and plants, to survive in freezing temperatures. While in solution, AFPs impart cryoprotection by creating a thermal hysteresis (TH), imparting ice recrystallization inhibition (IRI), and providing dynamic ice shaping (DIS). To leverage these ice-modulating effects of AFPs in other scenarios, a range of icing assays were performed with AFPs to investigate how AFPs interact with ice formation when tethered to a surface. In this work, we studied ApAFP752, an AFP from the beetle Anatolica polita, and first investigated whether removing the fusion protein attached during protein expression would result in a difference in freezing behavior. We performed optical microscopy to examine ice-crystal shape, micro-structure, and the recrystallization behavior of frozen droplets of AFP solutions. We developed a surface chemistry approach to tether these proteins to glass surfaces and conducted droplet-freezing experiments to probe the interactions of these proteins with ice formed on those surfaces. In solution, ApAFP752 did not show any DIS or TH, but it did show IRI capabilities. In surface studies, the freezing of AFP droplets on clean glass surfaces showed no dependence on concentration, and the results from freezing water droplets on AFP-decorated surfaces were inconclusive.en_US
dc.description.sponsorshipGoddard Space Flight Center.en_US
dc.description.tableofcontentsAbstract .......................................................................................................................................................... ii Figures ............................................................................................................................................................ iv Preface ............................................................................................................................................................ vi 1 Introduction ............................................................................................................................................ 1 1.1 Background ..................................................................................................................... 1 1.2 Objectives ........................................................................................................................ 4 1.3 Approach ......................................................................................................................... 5 2 Experimental Methods......................................................................................................................... 6 2.1 Materials ......................................................................................................................... 6 2.2 Antifreeze protein (AFP) and ice microstructure study ................................................. 6 2.3 Ice recrystallization inhibition (IRI) studies via splat assay .......................................... 7 2.4 Dynamic ice shaping (DIS) ............................................................................................. 7 2.5 AFP solution droplet freezing in oil solutions ................................................................ 8 2.6 Freezing AFP droplets on glass surfaces ....................................................................... 8 2.7 Attaching AFPs to glass surfaces ................................................................................... 9 2.8 Attaching AFPs to surfaces: surface characterization ................................................ 10 2.9 Water-droplet-freezing experiments on surfaces with AFP ......................................... 10 3 Results ..................................................................................................................................................12 3.1 Effect of ApAFP752 on the microstructure of polycrystalline ice ............................... 12 3.2 Splat assay IRI with various AFPs ................................................................................ 13 3.3 DIS with various AFPs ................................................................................................... 14 3.4 Pseudohomogeneous freezing of ApAFP752 solutions ............................................. 17 3.5 Heterogeneous freezing of ApAFP752 solutions on clean glass surfaces ................ 18 3.6 Surface characterization for ApAFP752-functionalized surfaces .............................. 20 3.7 Heterogeneous freezing of water on functionalized surfaces .................................... 24 4 Discussion ............................................................................................................................................ 26 5 Conclusions .......................................................................................................................................... 28 References ................................................................................................................................................... 29 Abbreviations ............................................................................................................................................... 32 Report Documentation Page .................................................................................................................... 34-
dc.format.extent42 pages / 5.16 MB-
dc.format.mediumPDF-
dc.language.isoen_USen_US
dc.publisherEngineer Research and Development Center (U.S.)en_US
dc.relation.ispartofseriesTechnical Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/CRREL TR-22-17-
dc.rightsApproved for Public Release; Distribution is Unlimited-
dc.sourceThis Digital Resource was created in Microsoft Word and Adobe Acrobat-
dc.subjectAntifreeze proteinen_US
dc.subjectCryoprotectionen_US
dc.subjectCrystallizationen_US
dc.subjectIce materialsen_US
dc.subjectIce mechanicsen_US
dc.subjectIce prevention and controlen_US
dc.subjectIce recrystallizationen_US
dc.subjectInterfacesen_US
dc.subjectSelf-assemblyen_US
dc.subjectSurface chemistryen_US
dc.titleInvestigations into the ice crystallization and freezing properties of the antifreeze protein ApAFP752en_US
dc.typeReporten_US
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