Impact of Incremental Sampling Methodology (ISM) on metals bioavailability
Clausen, Jay L.; Levitt, Laura.; Cary, Timothy J.; Parker, Nancy.; Beal, Samuel A.; Bednar, Anthony J.; Rosado, Dale.; Catt, Michael.; Armstrong, Kristie C.; Hayes, Charolett A.; Swope, Brandon.; Colvin, Marienne.; Sorensen, Kara.; Georgian, Thomas.
Abstract: This study assessed the impact of the incremental sampling methodology (ISM) on metals bioavailability through a series of digestion and in vivo experiments. These tests used Eisenia fetida and Lolium rigidum in both milled and unmilled loam and sand soil containing antimony, copper, lead, and zinc obtained from Donnelly Training Area, Alaska. No significant differences in metal levels were evident between milled and unmilled soil for E. fetida, and uptake of lead by L. rigidum in sand yielded lead recoveries comparable with Method 3050 analysis of soil. In contrast, L. rigidum grown in loam had much lower recoverable lead. Milling of the soil as part of the ISM process had no significant impact on the lead species distribution. In comparison with Method 3050, the alternative digestion tests involving the use of glycine; oxalate; ethylenediaminetetraacetic acid (EDTA); or alternative digestion procedures, such as the synthetic precipitation leaching procedure (SPLP) and the toxicity characteristic leaching procedure (TCLP), yielded lower recoveries of lead for all soil particle sizes and soil types. Diffusive gradient in thin films experiments yielded metal concentrations positively correlated with E. fetida concentrations. The physiologically based extraction technique (PBET) positively correlated with bulk soil concentrations and E. fetida tissue concentrations for all soils evaluated.
United States. Army. Corps of Engineers.Engineer Research and Development Center (U.S.)Cold Regions Research and Engineering Laboratory (U.S.)Environmental Laboratory (U.S.)U.S. Army Environmental Command.
Metals; Bioavailability; Soils--Sampling; Soil pollution; Toxicology
ERDC ; TR-16-4