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Title: Development of environmental health criteria for insensitive munitions : aquatic ecotoxicological exposures using 2,4-dinitroanisole
Authors: U.S. Army Institute of Public Health.
BAE SYSTEMS Ordnance Systems, Inc.
Badger Technical Services.
Strategic Environmental Research and Development Program (U.S.)
Kennedy, Alan James, 1976-
Lounds, Christopher D.
Melby, Nicolas L.
Laird, Jennifer G.
Winstead, Bob.
Brasfield, Sandra M.
Johnson, Mark S.
Keywords: 2,4 dinitroanisole
Acute toxicity testing
Aquatic ecotoxicological exposures
Chronic toxicity testing
Insensitive munitions
Strategic Environmental Research and Development Program
Publisher: Environmental Laboratory (U.S.)
Engineer Research and Development Center (U.S.)
Series/Report no.: ERDC/EL TR ; 13-2.
Description: Technical Report
Abstract: Insensitive munition formulation (IMX)-101 consists of 2,4-dinitroanisole (DNAN), 3-nitro-1,2,4-triazol-5-one (NTO), and nitroguanidine (NQ). While general aquatic ecotoxicological information is available for two of the IMX constituents (NTO and NQ), such data are not known to be available for DNAN. Thus, acute and chronic aquatic toxicity bioassays were conducted using standard fish (Pimephales promelas) and invertebrate (Ceriodaphnia dubia) models. Chemical analysis of test water indicated that DNAN concentrations were relatively stable during the bioassays. Acute toxicity was similar for the two species tested, with 48-hr lethal median concentrations (LC50) ranging from 37 to 42 mg/L DNAN. Chronic toxicity tests indicated that fish survival (7-day LC50 = 10 mg/L) was significantly more sensitive to DNAN relative to the invertebrate (no significant impact on survival at 24 mg/L). However, the reproduction endpoint for the invertebrate was significantly more sensitive to DNAN than survival. When assessing the most sensitive chronic endpoints, the two test species indicated similar chronic toxicity, with lowest observable adverse impacts ranging from 10 to 12 mg/L DNAN and median effects on sublethal endpoints (growth, reproduction) ranging from 11 to 15 mg/L DNAN. Chronic no-effect concentrations ranged from approximately 6 to 8 mg/L DNAN.
Rights: Approved for public release; distribution is unlimited.
Appears in Collections:Technical Report

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