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Title: Application of Sister Chromatid Exchange in marine polychaetes to Black Rock Harbor sediment : laboratory documentation phase
Authors: Environmental Research Laboratory (Narragansett, R.I.)
United States. Environmental Protection Agency.
Field Verification Program (Aquatic Disposal)
Pesch, Gerald G.
Mueller, Cornelia.
Pesch, Carol E.
Heltshe, James.
Schauer, Paul S.
Keywords: Marine pollution
Aquatic pollution
Genetic effects
Sister Chromatid Exchange
Dredged material
Dredging spoil
Biological assay
Water quality
Environmental effects
Black Rock Harbor
Publisher: Environmental Laboratory (U.S.)
Engineer Research and Development Center (U.S.)
Description: Technical Report
Abstract: This report presents an evaluation of the applicability of the cytogenetic technique of sister chromatid exchange (SCE) to measure genotoxic effects of highly contaminated dredged material, and the degree of variability and reproducibility inherent in the procedure. This project is part of the US Environmental Protection Agency/Corps of Engineers Field Verification Program. The SCE technique was applied to Nephtys incisa, an infaunal polychaete dominant in the benthic community at the Central Long Island Sound disposal site. The SCE response was measured in N. incisa exposed to suspended particulate and bedded phases of Black Rock Harbor (BRH) sediment in the laboratory. Neanthes arenaceodentata, a surrogate species, was tested in parallel to N. incisa. With the exception of one treatment in one experiment, the worm chromosomes were uniformly nonresponsive to BRH sediment. Replicate treatments within an experiment did not differ significantly for N. arenaceodentata. Differences between experiments and between species within an experiment were found. The reasons for the differences are not known. Differences in ability to metabolize polynuclear aromatic hydrocarbons, found in high concentrations in BRH sediments, is a likely but speculative reason. Clearly, additional research is needed before SCE could be used for routine testing. This investigation is the first phase in developing field-verified bioassessment evaluations for the Corps of Engineers and the US Environmental Protection Agency regulatory program for dredged material disposal. This report is not suitable for regulatory purposes; however, appropriate assessment methodologies that are field verified will be available at the conclusion of this program.
Rights: Approved for public release; distribution is unlimited.
Appears in Collections:Technical Report

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