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Please use this identifier to cite or link to this item: https://hdl.handle.net/11681/6351
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dc.contributor.authorTheriot, Edwin A.en_US
dc.contributor.authorGunner, Haim B., 1924-en_US
dc.contributor.authorKees, Stewart Lamar, 1958-1997en_US
dc.creatorUniversity of Massachusetts Amhersten_US
dc.creatorAquatic Plant Control Research Program (U.S.)en_US
dc.creatorU.S. Army Engineer Waterways Experiment Stationen_US
dc.date.accessioned2016-03-23T19:54:53Zen_US
dc.date.available2016-03-23T19:54:53Zen_US
dc.date.issued1996-09en_US
dc.identifier.govdocTechnical Report A-96-9en_US
dc.identifier.urihttp://hdl.handle.net/11681/6351en_US
dc.descriptionTechnical Reporten_US
dc.description.abstractThe submersed aquatic plants Myriophyllum spicatum L. (Eurasian watermilfoil) and Hydrilla verticillata (L. fil.) Royle (hydrilla) are a nuisance in waterways of the United States. Biological control with plant pathogens is a proven method for the management of aquatic plants. This study describes the identification, isolation, and characterization of lectins of Eurasian watermilfoil and hydrilla as a potential mechanism for specific attachment of fungal plant pathogens to the target plants. Lectins specific for α-L-fucose were isolated from both Eurasian watermilfoil and hydrilla. The watermilfoil lectin is a Type I lectin having a monomeric subunit molecular weight of 48 kilodaltons (kda) and exists in both dimeric and tetrameric form. The hydrilla lectin is a Type II lectin composed of two monomeric subunits having molecular weights of 67 and 50 kda, which persist as a dimer and a tetramer, respectively. Two fungal pathogens of Eurasian watermilfoil and two fungal pathogens of hydrilla were evaluated for their ability to attach and infect the target species and for their capacity to be agglutinated by physiological concentrations of purified lectin from each plant species. Findings of this study support the theory that microbial recognition by plant lectins is a mediator of host defense. This study also reports 16 new potential pathogens against hydrilla and 15 against Eurasian watermilfoil.en_US
dc.description.sponsorshipAquatic Plant Control Research Program (U.S.)en_US
dc.description.sponsorshipUnited States. Army. Corps of Engineersen_US
dc.description.tableofcontentsPreface.........................v 1-Introduction.........................1 Background.........................1 Purpose.........................4 2-Materials and Methods.........................5 Experimental Plant Material.........................5 Culture Media.........................5 Fungal Cultures.........................5 Rapid Screening Bioassay.........................6 Lectin Isolation, Purification, and Characterization.........................6 Host Penetration Specificity.........................8 Lectin Activity Assays.........................10 Statistical Methods.........................11 3-Results.........................12 Rapid Screening Bioassay.........................12 Lectin Isolation, Purification, and Characterization.........................12 Host Penetration Specificity.........................14 Lectin Activity Assays.........................15 4-Discussion.........................19 5-Conclusions.........................23 References.........................24 Figures 1-15 Appendix A: Media Formulations.........................A1 SF 298en_US
dc.format.extent51 pages/7.98 MBen_US
dc.format.mediumPDFen_US
dc.language.isoen_USen_US
dc.publisherU.S. Army Engineer Waterways Experiment Stationen_US
dc.relationhttp://acwc.sdp.sirsi.net/client/en_US/search/asset/1042369en_US
dc.relation.ispartofseriesTechnical Report (Aquatic Plant Control Research Program (U.S.)) ; no.Technical Report A-96-9en_US
dc.rightsApproved for public release; distribution is unlimiteden_US
dc.sourceThis Digital Resource was created from scans of the Print Resourceen_US
dc.subjectPlant lectinsen_US
dc.subjectPlant pathogensen_US
dc.subjectSubmersed aquatic plantsen_US
dc.subjectEurasian watermilfoilen_US
dc.subjectHydrillaen_US
dc.subjectAquatic Plant Control Research Program (U.S.)en_US
dc.titleSpecific association of plant pathogens with submersed aquatic plantsen_US
dc.typeReporten_US
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