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|Title:||Simplified concepts in spectroscopy and photochemistry|
|Authors:||Installation Restoration Research Program (U.S.)|
|Publisher:||Environmental Laboratory (U.S.)|
Engineer Research and Development Center (U.S.)
Abstract: Spectrophotometric techniques are powerful tools for determining both qualitative (including structural) and quantitative changes in organic and inorganic compounds, and these techniques are particularly useful for studying: concentration changes; solvent effects; the excited states of certain molecules; trends in ultraviolet (UV) and visible (VIS) spectral changes (due to such structural changes as the addition of electron donating or withdrawing groups to aromatic compounds or to the extension of pi system conjugation); the course of certain organic chemical reactions (such as in cis-trans isomerization or protein dye conjugation); steps in separation and purification by other analytical procedures (such as column chromatography); and use of UV and VIS stopped-flow technique for measurements of reaction rates in the optimization of analytical assays. This paper attempts to provide sufficient discussion of theoretical concepts for practical applications of spectroscopy and photochemistry to be more easily understood. However, the emphasis is on practical application, particularly through showing representative uses and examples of UV and VIS techniques with organic molecules.
|Rights:||Approved for public release; distribution is unlimited.|
|Appears in Collections:||Technical Report|