Please use this identifier to cite or link to this item: https://hdl.handle.net/11681/45961
Full metadata record
DC FieldValueLanguage
dc.contributor.authorWhitecloud, Simone S.-
dc.contributor.authorVermeulen, Holly H.-
dc.contributor.authorLichtner, Franz J.-
dc.contributor.authorPodpora, Nadia A.-
dc.contributor.authorCooke, Timothy J.-
dc.contributor.authorWilliams, Christopher R.-
dc.contributor.authorMusty, Michael-
dc.contributor.authorMacAllister, Irene E.-
dc.contributor.authorDorvee, Jason R.-
dc.creatorCold Regions Research and Engineering Laboratory (U.S.)-
dc.date.accessioned2022-11-10T14:48:44Z-
dc.date.available2022-11-10T14:48:44Z-
dc.date.issued2022-11-
dc.identifier.govdocERDC/CRREL TR-22-25-
dc.identifier.urihttps://hdl.handle.net/11681/45961-
dc.identifier.urihttp://dx.doi.org/10.21079/11681/45961-
dc.descriptionTechnical Reporten_US
dc.description.abstractPlants emit a bouquet of volatile organic compounds (VOCs) in response to both biotic and abiotic stresses and, simultaneously, eavesdrop on emit-ted signals to activate direct and indirect defenses. By gaining even a slight insight into the semantics of interplant communications, a unique aware-ness of the operational environment may be obtainable (e.g., knowledge of a disturbance within). In this effort, we used five species of plants, Arabidopsis thaliana, Panicum virgatum, Festuca rubra, Tradescantia zebrina, and Achillea millefolium, to produce and query VOCs emitted in response to mechanical wounding and light cycles. These plants provide a basis for further investigation in this communication system as they span model organisms, common house plants, and Arctic plants. The VOC com-position was complex; our parameter filtering often enabled us to reduce the noise to fewer than 50 compounds emitted over minutes to hours in a day. We were able to detect and measure the plant response through two analytical methods. This report documents the methods used, the data collected, and the analyses performed on the VOCs to determine if they can be used to increase environmental awareness of the battlespace.en_US
dc.description.sponsorshipUnited States. Army. Corps of Engineers.en_US
dc.description.tableofcontentsAbstract ................................................................................................................................................... ii Figures and Tables ................................................................................................................................. iv Preface .................................................................................................................................................... vi 1 Introduction ..................................................................................................................................... 1 1.1 Background ..................................................................................................................... 1 1.2 Objectives ........................................................................................................................ 4 1.3 Approach ......................................................................................................................... 4 2 Methods and Materials ................................................................................................................. 5 2.1 Plant material .................................................................................................................. 5 2.1.1 Plant species ................................................................................................................... 5 2.1.2 Plant propagation ............................................................................................................ 5 2.2 Volatile collection ............................................................................................................ 8 2.2.1 Collection apparatus construction ................................................................................. 8 2.2.2 Adsorption methods ...................................................................................................... 10 2.2.3 Volatile collection methods ........................................................................................... 12 2.3 Volatile analysis ............................................................................................................ 14 2.3.1 GC-FID method .............................................................................................................. 14 2.3.2 TD-GC-MS method ......................................................................................................... 14 2.4 Statistical analysis ........................................................................................................ 15 2.4.1 GC-FID data ................................................................................................................... 15 2.4.2 TD-GC-MS data .............................................................................................................. 17 3 Results and Discussion ................................................................................................................ 18 3.1 Plant wound response (GC-FID) ................................................................................... 18 3.1.1 Manual compilation and alignment ............................................................................. 18 3.1.2 Compilation and alignment with GCalignR .................................................................. 19 3.2 P. virgatum wounding response (TD-GC-MS) ............................................................... 22 3.3 A. thaliana sunset and sunrise response .................................................................... 26 4 Conclusions and Recommendations ......................................................................................... 30 References ............................................................................................................................................ 32 Appendix: Plant Volatile Collection and Analysis with Gas Chromatography Mass Spectroscopy Flame Ionization Detector in Response to Wounding ..................................... 36 Acronyms and Abbreviations .............................................................................................................. 44 Report Documentation Page .............................................................................................................. 45-
dc.format.extent53 pages / 2.03 MB-
dc.format.mediumPDF-
dc.language.isoen_USen_US
dc.publisherEngineer Research and Development Center (U.S.)en_US
dc.relation.ispartofseriesTechnical Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/CRREL TR-22-25-
dc.rightsApproved for Public Release; Distribution is Unlimited-
dc.sourceThis Digital Resource was created in Microsoft Word and Adobe Acrobat-
dc.subjectBiosensoren_US
dc.subjectEnvironmental awarenessen_US
dc.subjectGases from plants--Detection;en_US
dc.subjectGC-FIDen_US
dc.subjectPlants--Effect of stress onen_US
dc.subjectPlant volatilesen_US
dc.subjectTD-GC-MSen_US
dc.subjectThermal desorptionen_US
dc.subjectPoraPak Qen_US
dc.titleUnderstanding plant volatiles for environmental awareness : chemical composition in response to natural light cycles and woundingen_US
dc.typeReporten_US
Appears in Collections:Technical Report

Files in This Item:
File Description SizeFormat 
ERDC-CRREL TR-22-25.pdf2.03 MBAdobe PDFThumbnail
View/Open