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https://hdl.handle.net/11681/45961
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DC Field | Value | Language |
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dc.contributor.author | Whitecloud, Simone S. | - |
dc.contributor.author | Vermeulen, Holly H. | - |
dc.contributor.author | Lichtner, Franz J. | - |
dc.contributor.author | Podpora, Nadia A. | - |
dc.contributor.author | Cooke, Timothy J. | - |
dc.contributor.author | Williams, Christopher R. | - |
dc.contributor.author | Musty, Michael | - |
dc.contributor.author | MacAllister, Irene E. | - |
dc.contributor.author | Dorvee, Jason R. | - |
dc.creator | Cold Regions Research and Engineering Laboratory (U.S.) | - |
dc.date.accessioned | 2022-11-10T14:48:44Z | - |
dc.date.available | 2022-11-10T14:48:44Z | - |
dc.date.issued | 2022-11 | - |
dc.identifier.govdoc | ERDC/CRREL TR-22-25 | - |
dc.identifier.uri | https://hdl.handle.net/11681/45961 | - |
dc.identifier.uri | http://dx.doi.org/10.21079/11681/45961 | - |
dc.description | Technical Report | en_US |
dc.description.abstract | Plants emit a bouquet of volatile organic compounds (VOCs) in response to both biotic and abiotic stresses and, simultaneously, eavesdrop on emit-ted signals to activate direct and indirect defenses. By gaining even a slight insight into the semantics of interplant communications, a unique aware-ness of the operational environment may be obtainable (e.g., knowledge of a disturbance within). In this effort, we used five species of plants, Arabidopsis thaliana, Panicum virgatum, Festuca rubra, Tradescantia zebrina, and Achillea millefolium, to produce and query VOCs emitted in response to mechanical wounding and light cycles. These plants provide a basis for further investigation in this communication system as they span model organisms, common house plants, and Arctic plants. The VOC com-position was complex; our parameter filtering often enabled us to reduce the noise to fewer than 50 compounds emitted over minutes to hours in a day. We were able to detect and measure the plant response through two analytical methods. This report documents the methods used, the data collected, and the analyses performed on the VOCs to determine if they can be used to increase environmental awareness of the battlespace. | en_US |
dc.description.sponsorship | United States. Army. Corps of Engineers. | en_US |
dc.description.tableofcontents | Abstract ................................................................................................................................................... ii Figures and Tables ................................................................................................................................. iv Preface .................................................................................................................................................... vi 1 Introduction ..................................................................................................................................... 1 1.1 Background ..................................................................................................................... 1 1.2 Objectives ........................................................................................................................ 4 1.3 Approach ......................................................................................................................... 4 2 Methods and Materials ................................................................................................................. 5 2.1 Plant material .................................................................................................................. 5 2.1.1 Plant species ................................................................................................................... 5 2.1.2 Plant propagation ............................................................................................................ 5 2.2 Volatile collection ............................................................................................................ 8 2.2.1 Collection apparatus construction ................................................................................. 8 2.2.2 Adsorption methods ...................................................................................................... 10 2.2.3 Volatile collection methods ........................................................................................... 12 2.3 Volatile analysis ............................................................................................................ 14 2.3.1 GC-FID method .............................................................................................................. 14 2.3.2 TD-GC-MS method ......................................................................................................... 14 2.4 Statistical analysis ........................................................................................................ 15 2.4.1 GC-FID data ................................................................................................................... 15 2.4.2 TD-GC-MS data .............................................................................................................. 17 3 Results and Discussion ................................................................................................................ 18 3.1 Plant wound response (GC-FID) ................................................................................... 18 3.1.1 Manual compilation and alignment ............................................................................. 18 3.1.2 Compilation and alignment with GCalignR .................................................................. 19 3.2 P. virgatum wounding response (TD-GC-MS) ............................................................... 22 3.3 A. thaliana sunset and sunrise response .................................................................... 26 4 Conclusions and Recommendations ......................................................................................... 30 References ............................................................................................................................................ 32 Appendix: Plant Volatile Collection and Analysis with Gas Chromatography Mass Spectroscopy Flame Ionization Detector in Response to Wounding ..................................... 36 Acronyms and Abbreviations .............................................................................................................. 44 Report Documentation Page .............................................................................................................. 45 | - |
dc.format.extent | 53 pages / 2.03 MB | - |
dc.format.medium | - | |
dc.language.iso | en_US | en_US |
dc.publisher | Engineer Research and Development Center (U.S.) | en_US |
dc.relation.ispartofseries | Technical Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/CRREL TR-22-25 | - |
dc.rights | Approved for Public Release; Distribution is Unlimited | - |
dc.source | This Digital Resource was created in Microsoft Word and Adobe Acrobat | - |
dc.subject | Biosensor | en_US |
dc.subject | Environmental awareness | en_US |
dc.subject | Gases from plants--Detection; | en_US |
dc.subject | GC-FID | en_US |
dc.subject | Plants--Effect of stress on | en_US |
dc.subject | Plant volatiles | en_US |
dc.subject | TD-GC-MS | en_US |
dc.subject | Thermal desorption | en_US |
dc.subject | PoraPak Q | en_US |
dc.title | Understanding plant volatiles for environmental awareness : chemical composition in response to natural light cycles and wounding | en_US |
dc.type | Report | en_US |
Appears in Collections: | Technical Report |
Files in This Item:
File | Description | Size | Format | |
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ERDC-CRREL TR-22-25.pdf | 2.03 MB | Adobe PDF | View/Open |