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dc.contributor.authorLance, Richard F., 1967--
dc.contributor.authorGuan, Xin.-
dc.identifier.govdocERDC/EL MP-21-9-
dc.descriptionMiscellaneous Paperen_US
dc.description.abstractAquatic environmental DNA (eDNA) surveys are sometimes impacted by polymerase chain reaction (PCR) inhibitors. We tested varying concentrations of different inhibitors (humic, phytic, and tannic acids; crude leaf extracts) for impacts on quantitative PCR (qPCR) assays designed for eDNA surveys of bighead and silver carp (Hypophthalmichthys nobilis and Hypophthalmichthys molitrix). We also tested for inhibition by high concentrations of exogenous DNA, hypothesizing that DNA from increasingly closely related species would be increasingly inhibitory. All tested inhibitors impacted qPCR, though only at very high concentrations — likely a function, in part, of having used an inhibitor-resistant qPCR solution. Closer phylogenetic relatedness resulted in inhibition at lower exogenous DNA concentrations, but not at relatively close phylogenetic scales. Inhibition was also influenced by the qPCR reporter dye used. Importantly, different qPCR assays responded differently to the same inhibitor concentrations. Implications of these results are that the inclusion of more than one assay for the same target taxa in an eDNA survey may be an important countermeasure against false negatives and that internal positive controls may not, in the absence of efforts to maximize inhibition compatibility, provide useful information about the inhibition of an eDNA assay.en_US
dc.description.sponsorshipGreat Lakes Restoration Initiative (U.S.)en_US
dc.description.sponsorshipUnited States. Army. Corps of Engineers.-
dc.format.extent16 pages / 1.04 MB-
dc.publisherEnvironmental Laboratory (U.S.)en_US
dc.publisherEngineer Research and Development Center (U.S.)-
dc.relation.ispartofseriesMiscellaneous Paper (Engineer Research and Development Center (U.S.)) ; no. ERDC/EL MP-21-9-
dc.relation.isversionofLance, Richard F., and Xin Guan. "Variation in inhibitor effects on qPCR assays and implications for eDNA surveys." Canadian Journal of Fisheries and Aquatic Sciences 77, no. 1 (2020): 23-33.
dc.rightsApproved for Public Release; Distribution is Unlimited-
dc.sourceThis Digital Resource was created in Microsoft Word and Adobe Acrobat-
dc.subjectMicrobial ecologyen_US
dc.subjectMolecular microbiologyen_US
dc.subjectEnvironmental monitoringen_US
dc.subjectPolymerase chain reactionen_US
dc.subjectAquatic habitatsen_US
dc.subjectBighead carpen_US
dc.subjectSilver carpen_US
dc.titleVariation in inhibitor effects on qPCR assays and implications for eDNA surveysen_US
Appears in Collections:Miscellaneous Paper

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