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Please use this identifier to cite or link to this item: https://hdl.handle.net/11681/32969
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dc.contributor.authorKennedy, Alan James, 1976--
dc.contributor.authorWu, Qihua-
dc.contributor.authorKremer, Kathryn-
dc.contributor.authorGibbons, Stephen-
dc.date.accessioned2019-06-10T13:59:39Z-
dc.date.available2019-06-10T13:59:39Z-
dc.date.issued2019-05-
dc.identifier.govdocERDC/EL SR-19-1-
dc.identifier.urihttps://hdl.handle.net/11681/32969-
dc.identifier.urihttp://dx.doi.org/10.21079/11681/32969-
dc.descriptionSpecial Report-
dc.description.abstractA fluorescence spectroscopy technique has been widely used for characterizing the fluorescence of nanomaterials. The technique provides fast and sensitive measurements that are applicable in many research and industrial areas. Obtained absorbance, excitation, and fluorescence emission spectra can be used to determine sample concentrations, binding kinetics of biomolecules, reaction mechanisms, and many more applications. This scientific operating procedure SOP provides general guidance for using fluorescence spectroscopy techniques with an instrument such as a HORIBA Scientific NanoLog® spectrofluorometer to determine fluorescence emission and UV-Vis-NIR absorbance of nanomaterials in solution. Procedures and recommendations of sample preparation, instrument preparation, sample analysis, and results analysis are briefly described. The procedure was tested on a variety of nanomaterial solutions.en_US
dc.description.sponsorshipEnvironmental Consequences of Nanotechnologies Program (U.S.)en_US
dc.description.tableofcontentsAbstract ................................................................................................................................................... ii Preface .................................................................................................................................................... iv Unit Conversion Factors ......................................................................................................................... v Acronyms and Abbreviations ................................................................................................................ vi 1 Introduction ..................................................................................................................................... 1 1.1 Background .................................................................................................................... 1 1.2 Objective ........................................................................................................................ 2 1.3 Approach ........................................................................................................................ 2 2 Terminology ..................................................................................................................................... 4 2.1 Related documents ....................................................................................................... 4 2.2 Definitions ...................................................................................................................... 4 3 Materials and Apparatus ............................................................................................................... 5 3.1 Materials ........................................................................................................................ 5 3.2 Apparatus ....................................................................................................................... 5 4 Procedure ........................................................................................................................................ 6 4.2 Apparatus preparation .................................................................................................. 6 4.2.1 Wavelength accuracy qualification ............................................................................... 6 4.2.2 Detector sensitivity validation ....................................................................................... 7 4.2.3 Other instrument qualification ...................................................................................... 8 4.3 Sample measurement ................................................................................................... 8 4.3.1 Fluorescence emission measurement .......................................................................... 8 4.3.2 UV-Vis-NIR absorbance measurement .......................................................................... 9 5 Reporting ....................................................................................................................................... 10 5.1 Analysis of fluorescence emission results ................................................................. 10 5.2 Analysis of absorbance results ................................................................................... 10 5.3 Quality Assurance (QA)/Quality Control (QC) considerations .................................... 10 References ............................................................................................................................................ 11 Report Documentation Page-
dc.format.extent21 pages / 1.007 Mb-
dc.format.mediumPDF-
dc.language.isoen_USen_US
dc.publisherEnvironmental Laboratory (U.S.)en_US
dc.publisherEngineer Research and Development Center (U.S.)-
dc.relation.ispartofseriesSpecial Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/EL SR-19-1-
dc.rightsApproved for Public Release; Distribution is Unlimited-
dc.sourceThis Digital Resource was created in Microsoft Word and Adobe Acrobat-
dc.subjectFluorescence emissionen_US
dc.subjectUV-Vis-NIR absorbanceen_US
dc.subjectNanostructured materialsen_US
dc.subjectFluorescence spectroscopyen_US
dc.titleDetermination of fluorescence emission and UV-Vis-NIR absorbance for nanomaterials solution using a HORIBA Scientific NanoLog® spectrofluorometeren_US
dc.typeReporten_US
Appears in Collections:Special Report

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