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Title: Determination of fluorescence emission and UV-Vis-NIR absorbance for nanomaterials solution using a HORIBA Scientific NanoLog® spectrofluorometer
Authors: Kennedy, Alan James, 1976-
Wu, Qihua.
Kremer, Kathryn.
Gibbons, Stephen.
Keywords: Fluorescence emission
UV-Vis-NIR absorbance
Nanostructured materials
Fluorescence spectroscopy
Publisher: Environmental Laboratory (U.S.)
Engineer Research and Development Center (U.S.)
Series/Report no.: Special Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/EL SR-19-1
Abstract: A fluorescence spectroscopy technique has been widely used for characterizing the fluorescence of nanomaterials. The technique provides fast and sensitive measurements that are applicable in many research and industrial areas. Obtained absorbance, excitation, and fluorescence emission spectra can be used to determine sample concentrations, binding kinetics of biomolecules, reaction mechanisms, and many more applications. This scientific operating procedure SOP provides general guidance for using fluorescence spectroscopy techniques with an instrument such as a HORIBA Scientific NanoLog® spectrofluorometer to determine fluorescence emission and UV-Vis-NIR absorbance of nanomaterials in solution. Procedures and recommendations of sample preparation, instrument preparation, sample analysis, and results analysis are briefly described. The procedure was tested on a variety of nanomaterial solutions.
Description: Special Report
Gov't Doc #: ERDC/EL SR-19-1
Rights: Approved for Public Release; Distribution is Unlimited
Size: 21 pages / 1.007 Mb
Types of Materials: PDF
Appears in Collections:Special Report

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