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https://hdl.handle.net/11681/32969| Title: | Determination of fluorescence emission and UV-Vis-NIR absorbance for nanomaterials solution using a HORIBA Scientific NanoLog® spectrofluorometer |
| Authors: | Kennedy, Alan James, 1976- Wu, Qihua. Kremer, Kathryn. Gibbons, Stephen. |
| Keywords: | Fluorescence emission UV-Vis-NIR absorbance Nanostructured materials Fluorescence spectroscopy |
| Publisher: | Environmental Laboratory (U.S.) Engineer Research and Development Center (U.S.) |
| Series/Report no.: | Special Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/EL SR-19-1 |
| Abstract: | A fluorescence spectroscopy technique has been widely used for characterizing the fluorescence of nanomaterials. The technique provides fast and sensitive measurements that are applicable in many research and industrial areas. Obtained absorbance, excitation, and fluorescence emission spectra can be used to determine sample concentrations, binding kinetics of biomolecules, reaction mechanisms, and many more applications. This scientific operating procedure SOP provides general guidance for using fluorescence spectroscopy techniques with an instrument such as a HORIBA Scientific NanoLog® spectrofluorometer to determine fluorescence emission and UV-Vis-NIR absorbance of nanomaterials in solution. Procedures and recommendations of sample preparation, instrument preparation, sample analysis, and results analysis are briefly described. The procedure was tested on a variety of nanomaterial solutions. |
| Description: | Special Report |
| Gov't Doc #: | ERDC/EL SR-19-1 |
| Rights: | Approved for Public Release; Distribution is Unlimited |
| URI: | https://hdl.handle.net/11681/32969 http://dx.doi.org/10.21079/11681/32969 |
| Size: | 21 pages / 1.007 Mb |
| Types of Materials: | |
| Appears in Collections: | Special Report |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ERDC-EL SR-19-1.pdf | 1.03 MB | Adobe PDF |  View/Open |