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Title: Assessing the sediment toxicity of Insensitive Munitions (IM) compounds using 10-day whole-sediment exposures with freshwater invertebrates; Scientific Operating Procedure series : characterization of IMX ecotoxicological effects
Authors: Lotufo, Guilherme R.
Farrar, J. Daniel
Keywords: Explosives, Military--Toxicity testing
Aquatic organisms
Freshwater invertebrates
Publisher: Environmental Laboratory (U.S.)
Engineer Research and Development Center (U.S.)
Series/Report no.: Special Report (Engineer Research and Development Center (U.S.)) ; no. ERDC/EL SR-18-9
Abstract: This document provides a standard method for assessing the sediment toxicity of insensitive munitions (IM) compounds using juveniles of the amphipod Hyalella azteca and larvae of the midge Chironomus dilutus. The 10-day test is conducted at 23 ± 1 °C in 300 mL glass beakers containing sediment spiked with an IM formulation or a single constituent. Each beaker receives 100 mL of spiked or control sediment, 175 mL of overlying water, and ten organisms. The endpoints are survival and growth. Five replicate beakers per treatment are used. To prevent excessive loss of IM compounds, the overlying water in the beakers should not be replaced during the 10-day exposure period. Water quality parameters are measured at the start, during, and at test termination. The tests are only valid if the mean survival for the control treatment is 70% or greater for C. dilutus and 80% or greater for H. azteca. Instructions and requirements are provided for handling test organisms before and at the initiation of the toxicity test, initiating and terminating whole-sediment exposures, maintaining appropriate test conditions, making necessary observations and water quality measurements, assessing the survival and growth endpoints, and obtaining samples for chemical analysis.
Description: Special Report
Gov't Doc #: ERDC/EL SR-18-9
Appears in Collections:Special Report

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