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Title: Sediment spiking methodologies for nanomaterial powders and aqueous suspensions for aquatic toxicity bioassays : Scientific Operating Procedure series : SOP-T-3
Authors: Coleman, Jessica G.
Kennedy, Alan James, 1976-
Stanley, Jacob K.
Rabalais, Lauren K.
Keywords: Nanotechnology
Nanostructured materials
Environmental toxicology--Testing
Biological assay
Publisher: Environmental Laboratory (U.S.)
Engineer Research and Development Center (U.S.)
Series/Report no.: Special Report (Engineer Research and Development Center (U.S.)) ; ERDC/EL SR-18-3
Abstract: Homogenous dispersions of engineered nanomaterials (ENMs) for use in bioassay media for exposures/characterizations are crucial in ecotoxicology assays of nanomaterials in order to obtain reliable and repeatable results. Further, selection of the method for spiking sediment should consider the functional feeding behavior of benthic organisms which impact the way they are exposed to ENMs in the sediment; therefore, this procedure provides step-by-step methods for spiking nanoparticle working stock suspensions into or onto the surface of sediment for bioassays based on test organism functional group. Users are directed to follow the section relevant to their testing need and parent material (e.g., aqueous based-ENMs or powder ENMs). If followed correctly, near-homogenous particle dispersions of the reference materials and a best case scenario for dispersion of test materials in sediment/test water should be achieved using this procedural guidance.
Description: Special Report
Gov't Doc #: ERDC/EL SR-18-3
Rights: Approved for Public Release; Distribution is Unlimited
Size: 27 pages/2.464 Mb
Types of Materials: PDF/A
Appears in Collections:Special Report

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