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https://hdl.handle.net/11681/22721| Title: | Invasive species management on military lands : clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 (CRISPR/Cas9)-based gene drives |
| Authors: | Gong, Ping. |
| Keywords: | Invasive species Biocontrol Genetic control CRISPR/Cas9 Gene drive Military lands Nonindigenous pests Pests--Biological control Genetics Military bases Environmental protection |
| Publisher: | Environmental Laboratory (U.S.) Engineer Research and Development Center (U.S.) |
| Series/Report no.: | ERDC/EL;SR-17-2 |
| Abstract: | Abstract: Applications of genetic engineering-based technologies to the control of invasive species are emerging as an important area of innovation. As a mechanism to spread the desired genes and associated traits into the target population, gene drive is recognized as a promising genetic biocontrol strategy to combat invasive species. Unlike existing technologies that depend on the cumbersome custom-making of new proteins for each DNA target, the CRISPR system uses RNA as its DNA-homing mechanism, which makes it low cost, high efficiency, easy to implement, and it dramatically shortens the design-build-test cycle for gene drive development. The CRISPR/Cas9 technology can alter multiple loci at the target gene that prevent mutations from blocking the spread of the drive. It can also be used to develop multiple types of gene drives such as precision, immunization and reversal drives to precisely target a specific subpopulation, protect a population from any future gene drive “invasions,” and overwrite previously released drives in case of unanticipated effects, respectively. Poised to become a self-sustaining, highly efficient, environmentally benign, and cost-effective alternative for invasive species control, CRISPR/Cas9-mediated gene drives can sweep a “deleterious” gene through a population exponentially faster than the normal Mendelian inheritance, and thus suppress or eradicate the target invasive species. |
| URI: | http://hdl.handle.net/11681/22721 http://dx.doi.org/10.21079/11681/22721 |
| Appears in Collections: | Special Report |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| ERDC-EL SR-17-2.pdf | 4.04 MB | Adobe PDF |  View/Open |