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Title: Culture and toxicity tests using Los Angeles District bioassay animals, 'Acanthomysis' and 'Neanthes'
Authors: Tatem, Henry E.
Portzer, A. Susan.
Keywords: Biological assay
Cultures (Biology)
Publisher: Environmental Laboratory (U.S.)
Series/Report no.: Miscellaneous Paper;EL-85-6
Abstract: Abstract: This study describes culture techniques and results of toxicant bioassays using the California mysid, Acanthomysis sculpta, the polychaete, Neanthes arenaceodentata, and the gulf coast mysid, Mysidopsis bahia. Acanthomysia sculpta was cultured successfully in the laboratory using methods previously developed for M, bahia. The M. bahia were cultured at 20 ppt salinity and 25°C; A. sculpta were cultured at 30.ppt salinity and 15°C. The mysids were tested with three toxicants: dodecyl sodium sulfate (DSS), also known as sodium laurel sulfate; mercury (Hg); and polychlorinated biphenyls (PCB). The LC50 value at 72 hr for DSS showed the California mysid (0.96 ppm) more sensitive to this standard toxicant than the gulf coast mysid (3.80 ppm). Results of the Hg bioassays indicated that the California mysid was more tolerant of Hg than the gulf coast mysid. The Hg LC50 at 72 hr for the California mysid was 8.6 ppb compared to 4.4 ppb for the gulf coast mysid. It appears that the California mysid, in comparison to the gulf coast mysid, was more sensitive to DSS, but less sensitive to Hg. Results from PCB (Aroclor 1254) bioassays showed relatively little difference between species (A. sculpta - 12.5 ppb; M. bahia - 14.2 ppb - LC50 at 72 hr). Neanthes arenaceodentata were held in the laboratory according to established procedures and exposed to only one toxicant, DSS. In comparison to the mysids, the polychaete (8.0 ppm - LC50 at 72 hr). was not as sensitive to DSS. The Section 103 Implementation Manual recommends the gulf coast mysid as an internal standard for dredged material bioassays. Results from this study indicate that both the California mysid and the polychaete are also acceptable bioassay animals, and could be used for dredged material or other toxicity testing.
Appears in Collections:Miscellaneous Paper

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