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|Title:||An improved RP-HPLC method for determining nitroaromatics and nitramines in water|
|Authors:||U.S. Army Toxic and Hazardous Materials Agency.|
Jenkins, Thomas F.
Miyares, Paul H.
Walsh, Marianne E.
High-performance liquid chromatography
|Publisher:||Cold Regions Research and Engineering Laboratory (U.S.)|
Engineer Research and Development Center (U.S.)
|Series/Report no.:||Special report (Cold Regions Research and Engineering Laboratory (U.S.)) ; 88-23.|
Abstract: A protocol was developed for determining nitroaromatic and nitramine explosives by reversed-phase, high-performance liquid chromatography on an LC-18 column. The method employs dilution of an aqueous sample 1:1 with methanol, filtration through a 0.5-~m Millex-SR filter, separation on the LC-18 column using a 1:1 water-methanol eluent, and determination by UV-254 nm. A careful comparison was made with an earlier standard protocol, which uses separation on an LC-8 column with a 50:38:12 watermethanol-acetonitrile eluent. Overall, the new procedure provides better separation for a wider range of analytes and equivalent recovery for all analytes tested. The new procedure is particularly effective at separating TNT from tetryl, and it allows analysis of water and soil extracts using a single column and eluent combination. Reporting limits for HMX, RDX, TNB, DNB, NB, TNT, 2,4-DNT, 2,6-DNT, o-NT, m-NT, p-NT and 2,4,5-TNT ranged from 4 to 15 ~g/L. A higher reporting limit of 44 ~g/L was obtained for tetryl, apparently because of some instability in the methanol-water matrix. Analytical recovery averaged 99%, with standard deviations ranging from 1.6 to 5.1 ~g/L (standard deviation for tetryl = 15.7 ~g/L). Note: The symbol ~ is used to indicate the presence of a mathematical or scientific symbol that could not be rendered by the character set of this system. The downloaded file will contain the missing symbols.
|Rights:||Approved for public release; distribution is unlimited.|
|Appears in Collections:||Special Report|
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|SR-88-23.pdf||1.77 MB||Adobe PDF|